bile salt export protein  (Boster Bio)

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Effect of L-FABP gene ablation on liver levels of key proteins involved in uptake, synthesis and storage of bile acid precursor cholesterol Liver homogenates of male mice were separated by SDS/PAGE and electroblotted on to nitrocellulose membranes as described in the Experimental section. The membranes were incubated with polyclonal antisera directed against proteins involved in cholesterol uptake (LDL receptor), efflux (SRB-1), synthesis (HMG-CoA reductase) and storage as cholesteryl esters (ACBP, ACAT-2 and ACAT-1). The amount of protein is expressed in arbitrary units (a.u.) with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): *, P <0.05; **, P <0.01. For cholesterol diet versus control diet: #, P <0.05; ###, P <0.001.

Article Snippet: Goat polyclonal anti-mouse BSEP (bile salt export pump protein) and goat polyclonal anti-human MRP2 (multidrug resistance associated protein 2) were from Santa Cruz Biotechnology; rabbit polyclonal anti-rat OATP-1 (organic anion transporting polypeptide 1) was from Alpha Diagnostic International (San Antonio, TX, U.S.A.).

Techniques: Incubation

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Proteins from liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against CYP7A1 (A), CYP27A1 (B) or SCP-x (C) as described in the Experimental section. The amount of protein is expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): **, P<0.01; ***, P<0.001. For cholesterol diet versus control diet: #, P<0.05; and ###, P<0.001.

Article Snippet: Goat polyclonal anti-mouse BSEP (bile salt export pump protein) and goat polyclonal anti-human MRP2 (multidrug resistance associated protein 2) were from Santa Cruz Biotechnology; rabbit polyclonal anti-rat OATP-1 (organic anion transporting polypeptide 1) was from Alpha Diagnostic International (San Antonio, TX, U.S.A.).

Techniques: SDS Page, Incubation

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Proteins from liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against cytosolic cholesterol/bile acid transport proteins such as L-FABP (A), SCP-2 (B), caveolin-1 (C), GST (D), or 3α-HSD (E) as described in the Experimental section. The amount of protein was expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): *, P<0.05; **, P<0.01; ***, P<0.001. For cholesterol diet versus control diet: ##, P<0.01; and ###, P<0.001.

Article Snippet: Goat polyclonal anti-mouse BSEP (bile salt export pump protein) and goat polyclonal anti-human MRP2 (multidrug resistance associated protein 2) were from Santa Cruz Biotechnology; rabbit polyclonal anti-rat OATP-1 (organic anion transporting polypeptide 1) was from Alpha Diagnostic International (San Antonio, TX, U.S.A.).

Techniques: SDS Page, Incubation

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Proteins from liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against canalicular bile salt translocase proteins such as BSEP (A) and MRP2 (B), or against basolateral/serosal bile salt translocase protein such as OATP-1 (C) as described in the Experimental section. The amount of protein is expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): *, P<0.05; **, P<0.01; ***, P<0.001. For cholesterol diet versus control diet, ###, P<0.001.

Article Snippet: Goat polyclonal anti-mouse BSEP (bile salt export pump protein) and goat polyclonal anti-human MRP2 (multidrug resistance associated protein 2) were from Santa Cruz Biotechnology; rabbit polyclonal anti-rat OATP-1 (organic anion transporting polypeptide 1) was from Alpha Diagnostic International (San Antonio, TX, U.S.A.).

Techniques: SDS Page, Incubation

Journal:

Article Title: Liver fatty-acid-binding protein (L-FABP) gene ablation alters liver bile acid metabolism in male mice

doi: 10.1042/BJ20050296

Figure Lengend Snippet: Liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against nuclear regulatory proteins SREBP-1 (A), PPARα (B), FXR (C), LXRα (D) or SHP (E) as described in the Experimental section. The amount of protein is expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): **, P<0.01, ***P<0.001. For cholesterol diet versus control diet: #, P<0.05; ##, P<0.01; and ###, P<0.001.

Article Snippet: Goat polyclonal anti-mouse BSEP (bile salt export pump protein) and goat polyclonal anti-human MRP2 (multidrug resistance associated protein 2) were from Santa Cruz Biotechnology; rabbit polyclonal anti-rat OATP-1 (organic anion transporting polypeptide 1) was from Alpha Diagnostic International (San Antonio, TX, U.S.A.).

Techniques: SDS Page, Incubation